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Background: The COVID-19 pandemic has been striking for three years and, despite the regular arise of new variants, populations are now widely immune and protected from severe symptoms. However, immunocompromised patients still have worse clinical outcomes, higher mortality and rarely develop effective immunity through vaccination or infection. Here, we studied the temporal distribution of infections, viral loads (VL) as well as the viral genetic diversity among an immunocompromised patient cohort, between January 2021 and September 2022. Method(s): Overall, 478 immunocompromised patients (solid organ transplant, HIV positive, cancer, autoimmune disease) and 234 controls (healthcare workers) from Pitie-Salpetriere and Bichat Claude-Bernard University hospitals (Paris, FRANCE) were diagnosed with SARS-CoV-2 infection by RT-qPCR. Whole genome sequencing was performed according to ARTIC protocol on Oxford Nanopore platform. All 712 full viral genomes were used to determine lineages and mapped to Wuhan-Hu-1 reference to produce a maximum likelihood phylogenetic tree (IQTree, 1000 bootstraps). Differences in temporal distributions of infections and VL were assessed using nonparametric statistical tests. Result(s): According to phylogenetic analysis, genomes from SARS-CoV- 2 infecting immunocompromised patients and those infecting healthy individuals are distributed in a similar way. No significant genetic differences can be observed between viral genomes from patients and controls within the different lineages. Temporal distribution of COVID-19 infections were also similar between immunocompromised patients and controls, with the exception of BA.2 variant for which controls were infected earlier (p< 0.001). VL were significantly lower in immunocompromised patients infected with Omicron variants (p=0.04). No differences in VL were observed for Alpha and Delta variants. Conclusion(s): At diagnosis, no intrinsic genetic divergence was observed in virus infecting immunocompromised patients compared to those circulating in the general population. Similarities in temporal distribution of infections between controls and patients suggest that these different groups become infected concomitantly. VL appeared to be lower for Omicron variants in immunocompromised patients. An earlier VL peak of Omicron and a testing of immunocompromised patients hospitalized once severe symptoms have appeared could indicate a delayed testing in these patients, once the replicative phase over. (Figure Presented).
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Background: In 2018 we reported the emergence of the new HIV-1 recombinant CRF94-02BF2 involved in a large transmission cluster of 49 French MSM mostly infected in 2016-2017. This CRF94 raised concerns of enhanced virulence. Prevention actions were undertaken in the area and population affected. This study reported the molecular and epidemiological evolution of this CRF94 until June 2022. Method(s): In 2021-2022, French sequence databases were screened for patients infected with HIV-1 subtype CRF94 or similar strain. HIV subtyping was confirmed by phylogenetic analysis of genes encoding both protease and reverse transcriptase (1070bps), and integrase (696bps) using IQ-Tree. Five whole genomes, related but distinct from CRF94, were obtained with the DeepChek assay Whole Genome kits. Recombination breakpoints were estimated using RDP4 and SimPlot. Mann-Whitney and LogRank tests were used for statistical analyses to compare patients' characteristics. Result(s): In June 2022, 49 new HIV-1 sequences were collected: 14 clustered with the 49 previous CRF94, 32 formed a new cluster next to but distinct from CRF94, and 3 strains could not be classified. Analysis of 5 whole genomes from the new cluster revealed a new recombinant, the CRF132-94B, mainly consisting of CRF94 which recombined with subtype B in the POL and accessory genes. Vif gene changed from the F2 to the B subtype. Both CRF94 and 132 clusters involved >95% of MSM, mostly infected < 1 year before diagnosis. However, there were differences: 97% were diagnosed in 2013-2019 for CRF94 vs 90% in 2020-2022 for CRF132. At time of diagnosis, 33% of patients infected with CRF94 knew the Prep vs 95% for CRF132. In the cluster CRF94, patients were older (34 vs 30 years, p=0.02), had higher viral loads (5.42 vs 4.42 log10 copies/Ml;p< 0.001), a lower CD4 cell counts (358 vs 508 /mm3, p=0.002). On treatment, the patients with the CRF94 reached viremia < 50 copies/Ml significantly later than those infected with CRF132 (p=0.0002). The prevention activities targeting the CRF94 cluster could explained the few patients infected with this strain after 2018. The CRF132 is mainly located in another Paris region area, but no specific transmission place has been identified. Conclusion(s): After 2019, the CRF94 spread seems greatly slowed down but the very close CRF132-94B has given birth to a new highly active cluster in 2020- 2022, despite the COVID social-distancing and a strong knowledge of the Prep. CRF132 appears to be less virulent perhaps due to the Vif gene change. Identified breakpoints positions of the new HIV-1 CRF132-94B. GenBank accession numbers of the five references : ON901787 to ON901791.
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Background: Immunocompromised hosts with prolonged SARS-CoV-2 infections have been associated with the emergence of novel mutations, especially in the Spike protein, a key target for vaccines and therapeutics. Here, we conducted a case-control study to measure the genetic diversity of SARSCoV- 2 and to search for immunocompromised-specific minority variants. Method(s): SARS-CoV-2-positive patients with lung/cardiac/kidney transplant, HIV-positive, or treated with high doses of corticosteroids for auto-immune diseases were considered as immunocompromised hosts. SARS-CoV-2-positive healthcare workers with no auto-immune disease were used as controls. Samples were analyzed by RT-qPCR at Pitie-Salpetriere and Bichat Claude-Bernard university hospitals (Paris, France). Samples with Cycle threshold < 30 were selected for SARSCoV- 2 whole-genome sequencing using Oxford Nanopore protocol. Raw sequence data were mapped onto the Wuhan-Hu-1 reference genome, and consensus sequences were produced to determine the lineage. Only sequences covering at least 95% at >=50X depth of the Spike gene were investigated. In-house algorithms were developed to identify all majority and minority mutations in Spike. We defined a minority variant when it was present in >=6% and < 50% of the reads;and a majority variant when it was present in >50%. Result(s): We sequenced SARS-CoV-2 genome from 478 COVID-19- positive immunocompromised patients and 234 controls. More minority non-synonymous mutations in Spike were detected in viruses from immunocompromised hosts, compared to viral genomes from controls, in both Delta (p=0.001) and Omicron (p< 0.001) lineages, but not in Alpha (p=0.66) (Figure 1). Interestingly, among the 52 patients infected with the Delta variant, we concomitantly detected at low frequencies the mutations H655Y, N764K, D796Y, in three patients (associated with different auto-immune disease), that are part of Omicron variants signature mutations. Similarly, some patients (n=7) infected by Omicron BA.1 lineage had R346T at low-frequency, later fixed in Omicron BA.4.6 and BQ.1.1 lineages. None of these mutations were observed in the viral genomes from controls. Conclusion(s): Here, we report a higher genetic diversity in Spike gene among SARS-CoV-2 sequences from immunocompromised hosts for Delta and Omicron lineages. These results suggest that immunocompromised patients are more likely to allow viral genetic diversification and are associated with a risk of emergence of novel SARS-CoV-2 variants. (Figure Presented).
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In 2017, the French National Health Authority (HAS) reassessed its human immunodeficiency virus (HIV) screening strategy and in 2018, the Joint United Nations Programme on HIV/AIDS (UNAIDS) set the goal of screening 95% of people living with HIV by 2030. The HAS recommends an approach based on target population and gives the general practitioner (GP) a key role in its implementation. It is therefore important to facilitate HIV testing by GPs and to reduce missed opportunities. To this end, a pilot study was conducted on a panel of 2,000 GPs over a 10-month period in 2020 in order to evaluate the impact of a pop-up displayed within prescription assistance software reminding about the frequency of targeted screening recommended by the HAS. The pop-up was displayed for patients with a history of sexually transmitted infection and/or hepatitis C and/or tuberculosis in the previous 12 months and without a known HIV serology or diagnosis. The impact was measured by comparing the prospective follow-up of consultations made during the "pilot" in 2020 with the retrospective follow-up of consultations made during the "pre-pilot" period in 2019. The results showed a significant increase in HIV serology prescriptions during the pilot study, despite the COVID-19 pandemic. Difficulties in objectively identifying target patient profiles and in organizing regular follow-ups to HIV testing were also revealed. This pop-up tool represents an additional means of facilitating the prescription of HIV testing by GPs.
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Serology studies provide limited information on immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). This cross-sectional study aimed to assess prevalence and determinants of anti-SARS-CoV-2 cellular immunity in a cohort of heart transplant (HT) recipients. All consecutive HT recipients followed-up at our outpatient clinic between February and June 2022 providing informed consent were included in this observational cross-sectional study. We quantified SARS-CoV-2 Spike (S)-reactive and Nucleocapsid (N)-reactive T cells using enzyme-linked immunospot assay. A positive response was defined as S or N reactivity >8 spots/2 × 105 lymphocytes. Clinical characteristics, laboratory data, immunosuppressive regimen and vaccination status were compared between patients with and without SARS-CoV-2 S-reactive T cells. Categorical variables were described as number (%) and continuous variables with median [IQR]. Among 201 patients (age 58 [45-65] years, 77% males, time since transplantation 51 months [24-81]), 97 (48%) exhibit S-specific T cells, of which 58 had in addition N-reactive T cells. CD4 and CD8 T lymphocyte count, glomerular filtration rate, immunosuppressive regimen were associated with T cell response (Table). Among patients with detectable SARS-Co-V-2 cellular immunity, numbers of S-reactive T cells were higher in patients who had detectable N-reactive T cells (277 vs 93 /106 T cells) (Figure). Our study provides new information on cellular immunity against SARS-CoV-2 in HT recipients. [ FROM AUTHOR] Copyright of Journal of Heart & Lung Transplantation is the property of Elsevier B.V. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)
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Background: Recent studies reported poor to moderate humoral response after two vaccine doses in heart transplant recipients (HTR). Currently, French healthcare authorities recommend 2 and 3 vaccine injections for transplant recipients with and without prior SARS-CoV-2 infection, respectively. This study aimed to evaluate level and durability of humoral immunity with this vaccination strategy. Methods: This single-center cohort study included HTR followed at Paris Bichat hospital between January 2020 and September 2021. Analyses were performed using automated immunoassays (Abbot) to quantify anti-spike IgG (cut-off ≥ 7.1 BAU/mL) and anti-nucleocapsid IgG (cut-off index > 0.49), respectively. Categorical variables were described as number (%) and continuous variables with median (IQR). Results: A total of 181 HTR (75.7% males, age 58 y [47-66]) transplanted between June 1990 and June 2021, with cardiomyopathy (n=95), coronary artery disease (n=61), valvular cardiomyopathy (n=19) or other transplant indications were included. Median time from transplantation to first vaccine dose was 4.2 y [1.8-6.6]. 143 HTR (79%) had no SARS-CoV-2 infection history (HTRn) and 38 (21%) contracted the infection (HTRi) (56% before and 42% after vaccination initiation). After 2 vaccine injections, anti-S IgG seroconversion was observed for only 16% (n=12/76) of HTRn. Overall, anti-S IgG titers were lower in HTRn than in HTRi (0.5 [0.2-2.6] vs 578 [1.4-4449] BAU/mL, respectively, p=0.0001). The 3rd vaccine dose enabled to obtain 42% (n=33/72) of seroconversion among HTRn with median anti-S titers of 3.2 BAU/mL [0.4-35.0]. Only half seroconverters HTRn reached the 260 BAU/mL cut-off chosen by French authorities to define vaccination efficacy. Interestingly, these patients seem to have a sustained humoral response 4 months after the 3rd dose. Conclusion: This study gives new insights on the effect of the 3rd vaccine dose in HTR with low rate of seroconversion and low titers of anti-S IgG but sustained humoral response when seroconversion occurs. Studies on vaccine efficacy against SARS-CoV-2 variants and cell-mediated immune response in this cohort are ongoing.
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Background: In 2020, France reported 2.7 million cases of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), making it the second most affected European country by the COVID-19 pandemic after the United Kingdom. However, dynamics of SARS-CoV-2 transmissions within France or between France and other countries remains partially characterized. We propose an analysis of these dynamics on multiple scales, from the continents to the French administrative regions. Methods: We produced 736 SARS-CoV-2 sequences from Ile-de-France (Paris area, France) and analyzed them concomitantly with GISAID deposited sequences to elucidate the origins and spread of the virus from January 2020 to December 2020. A total of 4,571 worldwide sequences, including 1,652 French sequences, constituted the final dataset. All sequences were selected to be representative of each country temporal distribution of SARS-CoV-2 to the week resolution. We used a maximum likelihood phylogenetic framework to estimate the most probable temporal and geographic spread of SARS-CoV-2 within France and worldwide. Depending on the geographical focus (France, Europe or worldwide), we pruned the tree accordingly in 1,000 independent replicates. Results: Phylogenetic analysis revealed that, during the 1st French epidemic wave (from March to May), the majority of viruses introduced to France came from North America (USA) and Europe (Spain, Italy, ?). France regularly transmitted to neighboring European countries: Belgium, Germany, Italy and United Kingdom. Contrary to the 1st wave, inter-country transmission events were limited to neighboring countries and intercontinental transmission were almost absent during the French 2nd wave (from September to November). At the French regions-scale, we observed that Ile-de-France (IDF) was the main source of infections for all other French regions during the 1st epidemic wave, with a minor participation of Provence-Alpes-Côte d'Azur (PACA). For the 2nd epidemic wave, PACA was the main source of infections for all other French regions, with a lower participation of IDF and other regions. Conclusion: Overall, our findings allow a more comprehensive representation of SARS-CoV-2 transmission chains related to and within France and the global temporal distribution of those events, in link with control measures applied during the whole 2020 period. IDF and PACA were the main hubs of transmissions in France for the 1st and the 2nd epidemic waves, respectively.
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SARS-CoV-2 is a coronavirus causing a globalized outbreak called COVID-19. SARS-CoV-2 transmission is associated with inhalation of contaminated respiratory droplets and could causes severe complications. Until today several "waves" of infections have been observed despite implementation of strict health policies. Decisions for such sanitary measures are based on population health monitoring. Unfortunately, for COVID-19, a significant proportion of individuals are asymptomatic but play a role in the virus transmission. To overcome these limitations, several strategies were developed including genome quantification in wastewater that could allow monitoring of the health status of population, since shedding of SARS-CoV-2 in patient stool is frequent. Wastewater-based epidemiology (WBE) was established and several countries implemented this approach to allow COVID-19 outbreak monitoring. In France, the OBEPINE project performed a quantitative analysis of SARS-CoV-2 in raw wastewater samples collected from major wastewater treatment plants (WWTP) since March 2020. In the greater Paris area 1101 samples (507 for five WWTP and 594 for sewer) were collected. This 16 months monitoring allows us to observe the outbreak dynamics. Comparison of WBE indicators with health data lead to several important observation; the good level of correlation with incidence rates, the average 3 days lead time, and the sensitivity (WBE change when incidence is > to 7/100000 inhabitants). We also compared the local monitoring (city level) with the regional monitoring, to help cluster identification. Moreover, variants of concern (VOC) emerged due to the selection pressure. We developed a specific RT-qPCR method targeting the deletion H69-V70 in the spike protein, using this deletion as a proxy of the B.1.1.7 presence in the wastewater. With this data we demonstrate the predominant role played by this strain in the third wave. All these results allow a better description and understanding of the pandemic and highlight the role of such WBE indicators.
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COVID-19 , SARS-CoV-2 , Disease Outbreaks , Humans , Respiratory Aerosols and Droplets , WastewaterABSTRACT
Déclaration de liens d’intérêts: M.A. Khuong-Josses : essais cliniques en qualité d’investigateur principal (GSK), interventions ponctuelles (ViiV). Les autres auteurs n’ont pas précisé leurs éventuels liens d’intérêts.
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Introduction La première vague d’infection COVID a entraîné une fermeture brutale des consultations de patients suivis pour des pathologies chroniques dont le VIH. Même si les ordonnances étaient systématiquement renouvelées en ville et si les consultations ont ré-ouvert rapidement, une inquiétude sur le risque d’interruption des traitements et du suivi a émergé parmi les équipes soignantes prenant habituellement en charge ces patients. Nous avons souhaité évaluer la fréquence et la cause des interruptions du traitement et du suivi parmi les patients de notre service. Matériels et méthodes Nous avons repris les dossiers et résultats de tous les patients vus entre mai et juin 2020. Les résultats des charges virales, traitement, dates de consultation et évènements sur la prise des traitements ont été recueillis. Certains patients ont été exclus de l’analyse : les patients elite controller et les patients ayant débuté un traitement depuis moins de 6 mois. Résultats Au total, 300 recueils ont pu être effectués. Il s’agissait de femmes dans 64 % des cas, l’âge médian des patients était de 46 ans, 71 % d’entre eux étaient d’origine d’Afrique subsaharienne, 54 % étaient des ouvriers, 23 % n’avaient pas d’emploi, 90 % avaient un titre de séjour/nationalité, et 95 % une prise en charge sociale. Sept patients ont été exclus de l’analyse car elite controller, début de traitement ou découverte récente. Sur les 293 patients analysés, 31 % recevaient des anti-intégrases, 33 % des inhibiteurs non-nucléosidiques et 20 % des inhibiteurs de protéase, 20 % recevaient une bithérapie, et 40 % des patients avaient une charge virale indétectable depuis plus de 10 ans, le reste depuis en moyenne 4 ans. Alors que 93,8 % des patients avaient une charge virale indétectable avant le confinement, seulement 85 % des patients gardaient une charge virale indétectable au contrôle après le confinement, différence statistiquement significative (p<0,0005). Au total 36 patients ont positivé leur charge virale. Parmi ces patients 22 % recevaient des inhibiteurs non-nucléosidiques. Le délai entre les dates de consultation pré-confinement et post confinement était en moyenne de 6,3 mois (SD±6), délai que l’on remarque en général dans le suivi standard mais l’écart-type est large. Les raisons de la rupture de traitement pour ces patients étaient un voyage pour 12 d’entre eux (33 %), un seul patient avait manqué d’ordonnance et un autre évoquait un problème de confidentialité. Les risques potentiels sont le développement de résistances, différents sans doute selon la classe du 3e agent et la transmission possible de l’infection aux partenaires. Conclusion Le premier confinement lié à la crise sanitaire a entraîné un échappement virologique nous incitant à rester attentifs aux risques de rupture de suivi chez nos patients en période de crise sanitaire. Un des risques majeurs reste le voyage, avec plusieurs de nos patients restés bloqués dans leur pays d’origine. Un des biais principal de cette étude est son temps de recueil probablement trop court, pouvant sous-estimer le nombre de patients non encore revus depuis la première vague. Notre étude se poursuit pour vérifier un retour à une charge virale indétectable pour les patients s’étant positivés après la V1, et assurer les prochains confinements au maximum en maintenant le suivi actif de nos patients.
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Introduction Les populations de sans domicile fixe ont un taux de mortalité supérieur à celui de la population générale. Ceci est en partie en lien avec des maladies chroniques non suivis, des problèmes d’addiction ainsi qu’une exposition plus forte aux maladies transmissibles, en particulier respiratoires. Nous avons réalisé une étude pour évaluer les taux d’attaque, d’hospitalisation et de mortalité liés à l’infection par le SARS-CoV-2 dans une population de résidents de centres d’hébergement pour sans-abri et du personnel à leur contact. Matériels et méthodes Une étude sérologique rétrospective a été réalisée sur l’ensemble des résidents et des membres du personnel de trois centres d’hébergement pour sans-abri entre mars et mai 2020 : 2 centres de lits halte soins santé (LHSS) et un dortoir de femmes. Nous avons inclus tous les adultes présents dans les centres d’hébergement ou décédés d’une infection avérée par le SARS-CoV-2. Les IgG anti-SARS-CoV-2 étaient détectés par le test ELISA « SARS-CoV-2 IgG Architect (Abbott) ». Un cas confirmé de SARS-CoV-2 était défini comme tout participant présentant une PCR ou une sérologie positive. Des sérologies de contrôle ont été prélevées quatre mois après la première sérologie positive. Résultats Nous avons inclus 100 résidents et 83 membres du personnel. Le taux de SARS-CoV-2 confirmé par PCR ou sérologie était de 72/100 (72,0 %) pour les résidents et de 17/83 (20,5 %) pour le personnel. Le taux d’hospitalisation chez les résidents était de 17/72 (25 %) et le taux de décès de 4/72 (5,6 %). Toutes les hospitalisations sauf une et tous les décès sont survenus chez des résidents des LHSS. Trente-quatre sur 68 (50 %) des résidents des LHSS présentaient au moins deux facteurs de risque de forme grave d’infection par le SARS-CoV-2. Les femmes hébergées dans le dortoir étaient plus jeunes, présentaient moins de comorbidité, avaient le taux d’attaque le plus élevé (90,6 %) et une morbidité-mortalité quasi nulle. Cinquante-deux sur 80 (63,4 %) des personnes ayant une première sérologie positive ont eu une sérologie de contrôle à 4 mois de la première sérologie et 8 mois environ de leur infection. Parmi eux, 44 (84,6 %) avaient conservé des sérologies positives. Conclusion Le taux d’attaque du SARS-CoV-2 était extrêmement élevé chez les résidents des centres d’hébergement pour sans-abri par rapport à la population générale. Le risque d’infection grave par le SARS-CoV-2 était fortement associé à la présence de comorbidités à un plus jeune âge. Cette population à risqué doit être considérée comme prioritaire dans les campagnes de vaccination dans l’accès aux logements individuels pour les plus vulnérables.
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OBJECTIVES: Molecular assays on nasopharyngeal swabs remain the cornerstone of COVID-19 diagnostic. The high technicalities of nasopharyngeal sampling and molecular assays, as well as scarce resources of reagents, limit our testing capabilities. Several strategies failed, to date, to fully alleviate this testing process (e.g. saliva sampling or antigen testing on nasopharyngeal samples). We assessed the clinical performances of SARS-CoV-2 nucleocapsid antigen (N-antigen) ELISA detection in serum or plasma using the COVID-19 Quantigene R (AAZ, France) assay. METHODS: Performances were determined on 63 sera from 63 non-COVID patients and 227 serum samples (165 patients) from the French COVID and CoV-CONTACT cohorts with RT-PCR confirmed SARS-CoV-2 infection, including 142 serum (114 patients) obtained within 14 days after symptoms' onset. RESULTS: Specificity was 98.4% (95% confidence interval [CI], 95.3 to 100). Sensitivity was 79.3% overall (180/227, 95% CI, 74.0 to 84.6) and 93.0% (132/142, 95% CI, 88.7 to 97.2) within 14 days after symptoms onset. 91 included patients had a sera and nasopharyngeal swabs collected in the same 24 hours. Among those with high nasopharyngeal viral loads, i.e. Ct value below 30 and 33, only 1/50 and 4/67 tested negative for N-antigenemia, respectively. Among those with a negative nasopharyngeal RT-PCR, 8/12 presented positive N-antigenemia;the lower respiratory tract was explored for 6 of these 8 patients, showing positive RT-PCR in 5 cases. CONCLUSION: This is the first evaluation of a commercially available serum N-antigen detection assay. It presents a robust specificity and sensitivity within the first 14 days after symptoms onset. This approach provides a valuable new option for COVID-19 diagnosis, only requiring a blood draw and easily scalable in all clinical laboratories.
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Rationale: Patients with chronic intestinal failure (CIF) have a high probability of long-term survival on HPN. In the general population, patients with COVID-19 can experience digestive symptoms. Taking into account CIF patients characteristics, we hypothesized that a COVID-19 infection can determine delayed diagnosis and care, more severe complications, and atypical clinical presentations in CIF population. The objective of this study was to establish the clinical presentation of COVID 19 infection in CIF patients and their outcome. Methods: A prospective and retrospective national multicenter study, including all CIF patients (HPN > 3 months) with confirmed diagnosis of COVID-19 infection (by lung CTscan or PCR) were included. All approved HPN centre for adults and children participated. Results: The Inclusion period was from the 01/02/ 2020 to 05/05/2020. Among approximately 1000 CIF adult patients and 500 children, 9 (7F/2M) adult patients with a median BMI of 20.8 (±3)kg/m2 were diagnosed with Covid-19 infection, none in pediatric population. The median age was 56 (±23)years. The cause of CIF was short bowel syndrome (7/9) and motility disorder (2/9) with a 5,9 (±6) years of HPN duration. COVID 19 was confirmed by the association of positive COVID-19 PCR and CTscan in 6 cases, a positive PCR performed in a private laboratory in 2 cases and typical pulmonary lesions on CT-scan despite a negative PCR in one case. Clinical symptoms firstly described were fever (5/9), anosmia (2/9), headache (2/9), dyspnea (5/9) and digestive symptoms (4/9). Six patients were hospitalized, and 2 of them required intensive care. Two patients presented severe complications;one hyperosmolar hyperglycemic state in a patient without previous diabetes diagnosis and one death probably due to a massive pulmonary embolism 1 month after COVID-19 infection in a context of pulmonary arterial hypertension. Conclusion: In the context of COVID pandemia, the prevalence of COVID 19 diagnosis in CIF adult population seems to be around 9/1000. Currently, no case was diagnosed in pediatric CIF patients. Since the initial presentation could be unusual in patients without known risk factors, expert centers should be alerted. As the infectious disease continues to spread around the world, a worldwide international would be necessary to deepen those data. Disclosure of Interest: None declared
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Declaration de liens d'interets: Les auteurs declarent ne pas avoir de liens d'interets. Copyright © 2020